@article {10.3844/ajessp.2009.33.40, article_type = {journal}, title = {Improvement of Biohydrogen Production under Increased the Reactor Size by C. acetobutylicum NCIMB 13357 }, author = {Alshiyab, Hisham Salem and Kalil, Mohd Sahaid and Hamid, Aidil Abdul and Yusoff, Wan Mohtar Wan}, volume = {5}, number = {1}, year = {2009}, month = {Feb}, pages = {33-40}, doi = {10.3844/ajessp.2009.33.40}, url = {https://thescipub.com/abstract/ajessp.2009.33.40}, abstract = {Problem statement: One of the main factors influenced the bacterial productivity and total yield of hydrogen is the partial pressure of produced gas. A novel solution to enhance the bacterial productivity was through reduction of gas pressure. Approach: Increasing the reactor size showed to enhance the bacterial production of hydrogen. Results: The technique of increasing reactor size resulted to enhance the hydrogen yield (YP/S) from 269 mL g-1 glucose utilized to maximum yield of 448 mL g-1 glucose utilized by using 125 mL and 2 L reactor size respectively. The hydrogen productivity was also enhanced from 71 mL-1 h-1 to maximum of 91 mL L-1 h-1 was obtained by using 125 mL and 1 L reactor size respectively. Biomass concentration was enhanced from 1.03 g L-1 to maximum of 1.68 g L-1 by using 125 mL and 2 L reactor size were used respectively, hydrogen yield per biomass (YP/X) of 267 mL g-1 L-1, biomass per substrate utilized (YX/S) of 0.336 and produced hydrogen in gram per gram of glucose utilized (YH2/s) of 0.04 when 2 L reactor size was employed. Conclusion: By using bigger reactor size, the effect of gaseous products in fermentation medium was reduced and enhanced both bacterial productivity and biomass concentration.}, journal = {American Journal of Environmental Sciences}, publisher = {Science Publications} }