@article {10.3844/ajavsp.2019.190.196, article_type = {journal}, title = {Effect of Rumen Culture Adaptation to Origanum vulgare L. Essential Oil on Rumen Methane and Fermentation}, author = {Embaby, Mohamed G. and Günal, Mevlüt and AbuGhazaleh, Amer}, volume = {14}, number = {3}, year = {2019}, month = {Sep}, pages = {190-196}, doi = {10.3844/ajavsp.2019.190.196}, url = {https://thescipub.com/abstract/ajavsp.2019.190.196}, abstract = {The main objective of this study was to investigate the impact of adding oregano essential oil (OEO) to adapted and unadapted rumen cultures on methane gas (CH4) production and rumen fermentation under in vitro condition. Rumen fluid for culture fermenters was collected from a Holstein cow located in Southern Illinois University farms. The adapted and unadapted rumen cultures were obtained from continuous culture fermenters fed a control diet, or control diet plus OEO at 250 mg day-1 for 10 days. After 10 days of adaptation, the fermenter’s contents were incubated in a 24 h gas production experiment. Treatments were unadapted culture, unadapted culture plus OEO, adapted culture and adapted culture plus OEO. The OEO was added to rumen cultures at the rate of 500 mg L-1. After 24-h of incubation, total gas production decreased (p}, journal = {American Journal of Animal and Veterinary Sciences}, publisher = {Science Publications} }