@article {10.3844/ajavsp.2016.55.60, article_type = {journal}, title = {The Effects of Subacute Ruminal Acidosis on Milk Fatty Acid Profile in Dairy Cattle}, author = {Mitchell, C. and ALZahal, O. and Or-Rashid, M.M. and Steele, M.A. and McBride, B.W.}, volume = {11}, number = {2}, year = {2016}, month = {Apr}, pages = {55-60}, doi = {10.3844/ajavsp.2016.55.60}, url = {https://thescipub.com/abstract/ajavsp.2016.55.60}, abstract = {The objective of this experiment was to examine the effect of Subacute Ruminal Acidosis (SARA) on milk fatty acid (FA) profiles with emphasis on odd-chain and branched-chain FA. Sixteen multiparous Holstein dairy cows (618±35 kg of body weight, 221±32 days in milk) were used in a randomized complete-block design. During week 1, all cows received a high-forage diet (90% chopped grass hay, 5% grain mix, 5% protein supplement, dry-basis; HF). During weeks 2 through 4, cows either continued receiving the HF diet or received a low-fiber diet (38% chopped grass hay, 57% grain mix, 5% protein supplement, dry-basis; LF). Milk yield and dry matter intake were recorded daily, milk samples were collected twice weekly and ruminal pH was continuously recorded during the last 48 h of each week. Statistical analysis was conducted on weekly measuring Proc Mixed of SAS accounting for repeated measurement. The model included the fixed effects of diet, week, their interaction and block. Helmert contrasts were used to describe the difference between treatments over time. The dietary treatment induced a significant difference over time between treatments (diet by week 1 Vs. average week 2, 3 and 4; p}, journal = {American Journal of Animal and Veterinary Sciences}, publisher = {Science Publications} }