@article {10.3844/ajavsp.2015.36.42, article_type = {journal}, title = {Influences of Feed Additives on in vitro Volatile Fatty Acid Production}, author = {Duncan, A. V. and Woldeghebriel, A. and Privott, C. and Carver, J. and Holmes, B. and Henson, J. and Worku, M.}, volume = {10}, number = {1}, year = {2014}, month = {Dec}, pages = {36-42}, doi = {10.3844/ajavsp.2015.36.42}, url = {https://thescipub.com/abstract/ajavsp.2015.36.42}, abstract = {This study investigated effects of the feed additives nitrate and fumarate alone or in combination on in vitro Volatile Fatty Acid (VFA) production. Rumen fluid was collected from Holstein-Friesian cattle averaging 650 kg in BW (body weight) offered 11.4 kg d-1 per animal of concentrate diet containing equal amounts of soybean meal, whole cottonseed and ground corn once a day with free access to grass hay. The feed additives used were nitrate, fumarate and a nitrate-fumarate combination. Concentrations of VFAs were measured using Gas Chromatography. The results of the study revealed that nitrate decreased (p}, journal = {American Journal of Animal and Veterinary Sciences}, publisher = {Science Publications} }