@article {10.3844/ajavsp.2012.126.135,
article_type = {journal},
title = {Influence of Heirloom Tomato Polyphenol Extracts on Expression of Inflammation Genes in Bovine},
author = {Gyenai, Kwaku and Mikiashvili, Nona and Ismail, Hamid and Worku, Mulumebet},
volume = {7},
number = {3},
year = {2012},
month = {Aug},
pages = {126-135},
doi = {10.3844/ajavsp.2012.126.135},
url = {https://thescipub.com/abstract/ajavsp.2012.126.135},
abstract = {The effect of tomato on diseases of economic importance continues to be of broad interest. To evaluate these relationships, the effect of crude extracts of Cherokee purple tomato on pro-inflammatory genes and cytokines were evaluated. Neutrophils from three Holstein Fresian cows were treated with Phosphate Buffered Saline (PBS) or E. coli O111-B4 Lipopolysaccharide (LPS). Treated cells were exposed to different concentrations of polyphenol extracts of fresh and heated Cherokee purple. Transcription of Cyclooxygenase-2 (Cox-2) and Tumor Necrosis Factor-alpha (TNF-α) was detected using real time PCR. Secretion of cytokines was evaluated using specific Enzyme-Linked Immunosorbent Assay (ELISA)’s for three pro-inflammatory genes. Transcription of Cox-2 and TNF-α in bovine neutrophils were found. No significant treatment effect of tomato polyphenols was found in the LPS treated neutrophils. TNF-α and Cox-2 expression in bovine neutrophils were modulated by polyphenol treatment. Variable concentration of polyphenol extracts had no effect on Cox-2 transcription. Gene expression analysis of TNF-α and Cox-2 mRNA showed significantly decreased transcription of Cox-2 in neutrophils exposed to polyphenol extracts (p},
journal = {American Journal of Animal and Veterinary Sciences},
publisher = {Science Publications}
}