@article {10.3844/ajassp.2009.1448.1452, article_type = {journal}, title = {Cross Reactivity between Dromedary Whey Proteins and IgG Anti Bovine α-Lactalbumin and Anti Bovine β-Lactoglobulin }, author = {Youcef, N. and Saidi, D. and Mezemaze, F. and El-Mecherfi, K. E. and Kaddouri, H. and Negaoui, H. and Chekroun, A. and Kheroua, O.}, volume = {6}, year = {2009}, month = {Aug}, pages = {1448-1452}, doi = {10.3844/ajassp.2009.1448.1452}, url = {https://thescipub.com/abstract/ajassp.2009.1448.1452}, abstract = {Problem statement: Our aim was to enhance the data on antigenic properties of dromedary whey proteins. Approach: The identification of the whey proteins was carried by SDS-page and Reversed phase high performance liquid chromatography (RP-HPLC). The cross-reactivity of dromedary whey proteins with IgG anti bovine β-lactoglobulin and anti bovine α-lactalbumin, obtained by immunisation of Balb/c mice, was carried out by ELISA. Results: The SDS-page showed the presence of band corresponding to α-lactalbumin and albumin serum; this was confirmed by the chromatogram obtained by RP-HPLC, where we detected a pick corresponding to α-lactalbumin. There was a cross reaction of dromedary whey proteins with IgG anti bovin α-lactalbumin but it was very weak with IgG anti boin β-lactoglobulin. Conclusion: We detected a cross reactivity between dromedary whey proteins and IgG anti bovin α-lactalbumin.}, journal = {American Journal of Applied Sciences}, publisher = {Science Publications} }