TY  - JOUR
AU  - Puah, Chiew Wei 
AU  - Choo, Yuen May 
AU  - Ma, Ah Ngan 
AU  - Chuah, Cheng Hock 
PY  - 2007
TI  - The Effect of Physical Refining on Palm Vitamin E (Tocopherol, Tocotrienol and Tocomonoenol) 
JF  - American Journal of Applied Sciences
VL  - 4
IS  - 6
DO  - 10.3844/ajassp.2007.374.377
UR  - https://thescipub.com/abstract/ajassp.2007.374.377
AB  - This study reports the effect of physical refining on palm vitamin E including α- tocopherol, α-, γ and α-tocotrienols as well as α- tocomonoenol. A method using HPLC with fluorescence detector using normal phase silica column is described. An isocratic elution with n-hexane/THF/2-propanol (1000:60:4, by vol.) as mobile phase was used. The structure of the α- tocomonoenol was determined using gas chromatography coupled with mass spectrometry. The composition of the vitamers were α-tocopherol (14-17%), α-tocotrienol (22-24%), γ- tocotrienol (49-53%), α- tocotrienol (6-7%) and α- tocomonoenol (3%) throughout the physical refining. The concentration of all vitamers in crude palm oil was 1273±18 ppm. The concentrations of all vitamers in degummed palm oil, bleached palm oil and deodorized palm oil were 1134±20 ppm, 1095±18 ppm and 1029±18 ppm, respectively. This method provides fast and valuable information with minimal analysis time and no sample pre-treatment.