Research Article Open Access

Molecular Characterization and Differentiation of Proteases Isolated from Different Aspergillus Fungal Species

Samia Abd Allah Abdal-Aziz1 and Safaa M. Ali1
  • 1 Genetic Engineering and Biotechnology Research Institute, Egypt
OnLine Journal of Biological Sciences
Volume 21 No. 1, 2021, 69-119


Submitted On: 19 October 2020 Published On: 11 February 2021

How to Cite: Abdal-Aziz, S. A. A. & M. Ali, S. (2021). Molecular Characterization and Differentiation of Proteases Isolated from Different Aspergillus Fungal Species. OnLine Journal of Biological Sciences, 21(1), 69-119.


A comparative molecular study of Aperigiullus protease using sequence of protease gene representative 25 different Aspergillus species (Aspergillus fumigatus, Aspergillus oryzae, Aspergillus flavus, Aspergillus novofumigatus, Aspergillus viridinutans, Aspergillus pseudotamarii, Aspergillus clavatus, Aspergillus sojae, Aspergillus fischeri, Aspergillus caelatus, Aspergillus novofumigatus, Aspergillus campestris, Aspergillus costaricaensis, Aspergillus candidus, Aspergillus neoniger, Aspergillus mulundensis, Aspergillus ibericus, Aspergillus niger, Aspergillus piperis, Aspergillus eucalypticola, Aspergillus vadensis, Aspergillus steynii, and Aspergillus lentulus) have been described for several properties. To summarise the Asperigillus protease correlation, alignment of multiple sequence and phylogenetic tree assembly were carried out. 59 Protease gene amino acid sequence used for the constructed phylogenetic tree that showed the specific protease clusters of several species of Aspergillus (Aspergillus oryzae, Aspergillus fumigatus, Aspergillus clavatus, Aspergillus niger, Aspergillus nidulans, Aspergillus viridinutans, Aspergillus flavus and Aspergillus fischeri). Protease gene amino acid sequences acquired from NCBI. The homology sequence level was done using amino acid sequence. Among these proteases, the distributions of the commonly observed motifs were analysed. Protease protein sequences indicate that the structure and enzymatic function are involved.

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  • Protease
  • Aspergillus
  • Sequence
  • Amino Acid
  • Phylogenetic