Research Article Open Access

Screening of Blast Resistance Genes from South Tapanuli Rice Cultivars, North Sumatra, Indonesia

Saleha Hannum1, Hesti Wahyuningsih1, Riyanto Sinaga1, Ummu Kulsum Hasibuan1 and Adrian Hartanto1
  • 1 Universitas Sumatera Utara, Indonesia
OnLine Journal of Biological Sciences
Volume 20 No. 2, 2020, 99-106

DOI: https://doi.org/10.3844/ojbsci.2020.99.106

Submitted On: 5 April 2020 Published On: 25 May 2020

How to Cite: Hannum, S., Wahyuningsih, H., Sinaga, R., Hasibuan, U. K. & Hartanto, A. (2020). Screening of Blast Resistance Genes from South Tapanuli Rice Cultivars, North Sumatra, Indonesia. OnLine Journal of Biological Sciences, 20(2), 99-106. https://doi.org/10.3844/ojbsci.2020.99.106

Abstract

Rice blast disease caused by a phytopathogenic fungus, Magnaporthe oryzae, has been considered as a major threat to global rice productivity, including Indonesia. South Tapanuli is a regency in North Sumatra, known for its intensive and significant rice production activities. A collection of 13 rice cultivars originating from the region, is claimed for its resistances against the rice blast disease based on the practices by the local farmers. Molecular screening of blast resistance (R) genes was initiated to the 13 accessions using the Polymerase Chain Reaction (PCR) targeting five blast resistance (R) genes, Pup1, Pi-37, Pi-d2, Pi-ta2, Pib and Pi-kh. Out of the 13 accessions, the Pi-d2, Pi-ta2 and Pib were detected in all cultivars, showing 100% of R gene frequency. The second dominant, Pup1 and Pi-37 gene frequencies were 76.9 and 61.5%, respectively while the least dominant, Pi-kh gene frequency was 15.4%. The number of R genes detected among cultivars was ranged between 3 to 6 genes. The genetic associations among thirteen germplasms were determined using unweighted pair group method with arithmetic mean (UPGMA) analysis. Cluster analysis revealed that the least blast-resistant cultivars: Putri Kembar, Silatian and Siganteng, were grouped into cluster 1, showing polymorphism for Pi-d2, Pi-ta2 and Pi-b. The high-resistant cultivars, Martabe-Sicondong and Sayuti, Sitolas, Pulo Raja, Pulo Pandan, Siporang and Pulo Manggis, were grouped into cluster 2 and 3, respectively. The medium blast-resistant cultivars, IR 64 and Sitampan were grouped into cluster 4, showing polymorphism for Pup1, Pi-d2, Pi-ta2 and Pi-b. These results indicated that the utilization of blast-resistant cultivars in North Tapanuli was supported by the presence of R genes.

  • 647 Views
  • 325 Downloads
  • 0 Citations

Download

Keywords

  • Blast Resistance Gene
  • Sumatra
  • PCR
  • Rice