Research Article Open Access

Utilization of Charge Transfer and Kinetic Methods for the Determination of Doripenem

Nora H. Al-Shaalan1
  • 1 ,
American Journal of Applied Sciences
Volume 9 No. 4, 2012, 510-518

DOI: https://doi.org/10.3844/ajassp.2012.510.518

Submitted On: 6 January 2012 Published On: 10 February 2012

How to Cite: Al-Shaalan, N. H. (2012). Utilization of Charge Transfer and Kinetic Methods for the Determination of Doripenem. American Journal of Applied Sciences, 9(4), 510-518. https://doi.org/10.3844/ajassp.2012.510.518

Abstract

Problem statement: This study presents rapid, uncomplicated and sensitive spectrophotometric techniques for the analysis of doripenem in both its pure and pharmaceutical forms. Approach: The main principle of the approaches and procedures that have been developed is the reaction of doripenem as an n-electron donor to ninhydrin and π-acceptors, such as tetracyanoethylene, 7,7,8,8-tetracyanoquinodimethane, 2,3,5,6-tetrachloro-1,4-benzoquinone, 2,3-dichloro-5,6-dicyano-1,4-benzoquinone and chloranilic acid. The wavelengths 580, 264, 320, 357, 375 and 465 nm was used for measuring the resulting complexes for ninhydrin, chlorine, chloranilic acid, DDQ, TCNQ and TCNE respectively. Results: The suggested procedures for the determination of doripenem were successfully put into practice with good recovery; the percent recovery ranged from 99.31-100.55. Benes-Hildebrand plots were also used to study the association constants and the free energy changes. Conclusion: The results of the CT method agree with those of the kinetic method and there is no significant difference between them.

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Keywords

  • Charge transfer
  • kinetic method
  • association constants
  • significant difference
  • chloranilic acid
  • energy changes
  • stability studies
  • aqueous solutions