Research Article Open Access

Moringa Oleifera Lam Mitigates Oxidative Damage and Brain Infarct Volume in Focal Cerebral Ischemia

Woranan Kirisattayakul1, Jintanaporn Wattanathorn1, Terdthai Tong-Un1, Supaporn Muchimapura1 and Panakaporn Wannanon1
  • 1 Khon Kaen University, Thailand
American Journal of Applied Sciences
Volume 9 No. 9, 2012, 1457-1463

DOI: https://doi.org/10.3844/ajassp.2012.1457.1463

Submitted On: 12 April 2012 Published On: 3 August 2012

How to Cite: Kirisattayakul, W., Wattanathorn, J., Tong-Un, T., Muchimapura, S. & Wannanon, P. (2012). Moringa Oleifera Lam Mitigates Oxidative Damage and Brain Infarct Volume in Focal Cerebral Ischemia. American Journal of Applied Sciences, 9(9), 1457-1463. https://doi.org/10.3844/ajassp.2012.1457.1463

Abstract

Problem statement: At present, the therapeutic outcome of cerebral ischemia is still not in the satisfaction level. Therefore, the preventive strategy is considered. Based on the protective effect against oxidative damage of Moringa oleifera Lam. Leaves extract, we hypothesized that this plant extract might protect against cerebral ischemia, one of the challenge problems nowadays. In order to test this hypothesis, we aimed to determine the protective effect of M.oleifera leaves extract in animal model of focal cerebral ischemia induced by permanent occlusion of right middle cerebral artery. Approach: Male Wistar rats, weighing 300-350 g, were orally given the extract once daily at doses of 100, 200 and 400 mg kg-1 BW at a period of 2 weeks, then, they were permanently occluded the right Middle Cerebral Artery (MCAO). The animals were assessed the cerebral infarction volume and oxidative damage markers including MDA level and the activities of SOD, CAT and GSHPx enzymes at 24 h after occlusion. Results: Rats subjected to M.oleifera extract at all doses used in this study significantly decreased brain infarct volume both at cortical and subcortical structures in accompany with the elevation of SOD activity in both hippocampus and striatum while only the rats exposed to the extract at doses of 100 and 400 mg kg-1 BW showed the increased GSHPx activity in hippocampus. No the changes were observed. Therefore, our results demonstrates the potential benefit of M.oleifera leaves to decrease oxidative stress damage and brain infarct volume. Conclusion: This study is the first study to demonstrate the neuroprotective effect against focal cerebral ischemia of M.oleifera leaves. It suggests that M.oleifera may be served as natural resource for developing neuroprotectant against focal cerebral ischemia. However, the precise underlying mechanism and possible active ingredient are still required further study.

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Keywords

  • Moringa oleifera
  • neuroprotective
  • cerebral ischemia
  • oxidative damage
  • brain infarct volume