Research Article Open Access

Screening of Lactic Acid Bacteria for Antifungal Activity against Aspergillus oryzae

Belal Jamal Muhialdin1 and Zaiton Hassan2
  • 1 , Afganistan
  • 2 ,
American Journal of Applied Sciences
Volume 8 No. 5, 2011, 447-451


Submitted On: 6 February 2011 Published On: 17 May 2011

How to Cite: Muhialdin, B. J. & Hassan, Z. (2011). Screening of Lactic Acid Bacteria for Antifungal Activity against Aspergillus oryzae. American Journal of Applied Sciences, 8(5), 447-451.


Problem statement: The growth of spoilage fungi have been a global concern because of the economy loses and the health hazard of the mycotoxins produced by the spoilage fungi. Approach: A total of 137 lactic acid bacteria isolated from Malaysian fruits and fermented foods were screened for antifungal activity using dual agar overlay method and well method against A. oryzae. Results: 23 isolates showed inhibition activity after 72 h incubation at 30°C. Supernatant of three isolates with strong antifungal activity was evaluated by well method and they inhibited the growth of the fungi at 30°C for 72 h. LAB supernatant reduced the mass growth of Aspergillus oryzae when incubated for 7 days at 30°C. The isolates were identified using API 50CH as Lactobacillus brevis G004, Lactobacillus fermentum Te007 and Pediococcus pentosaceus Te010. Conclusion: The three isolates studied inhibited the growth of the mycelia and conidia germination of the fungi which indicate the possibility of using LAB isolates as biopreservative.

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  • A. oryzae
  • antifungal activity
  • API 50CHL
  • Lactic Acid Bacteria (LAB)
  • global concern
  • antifungal activity
  • isolates inhibited
  • spoilage moulds