Research Article Open Access

Antiproliferative Effects of Alkaloids Isolated from the Tuber of Stephania venosa via the Induction of Cell Cycle Arrest in Mammalian Cancer Cell Lines

Sirinapa Nantapap1, Chatchanok Loetchutinat1, Puttinan Meepowpan1, Narong Nuntasaen1 and Wilart Pompimon1
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American Journal of Applied Sciences
Volume 7 No. 8, 2010, 1057-1065

DOI: https://doi.org/10.3844/ajassp.2010.1057.1065

Submitted On: 29 July 2010 Published On: 31 August 2010

How to Cite: Nantapap, S., Loetchutinat, C., Meepowpan, P., Nuntasaen, N. & Pompimon, W. (2010). Antiproliferative Effects of Alkaloids Isolated from the Tuber of Stephania venosa via the Induction of Cell Cycle Arrest in Mammalian Cancer Cell Lines. American Journal of Applied Sciences, 7(8), 1057-1065. https://doi.org/10.3844/ajassp.2010.1057.1065

Abstract

Problem statement: S. venosa (Menispermaceae) is used in traditional medicine. The constituents of S. venosa belonging to showed remarkable cytotoxic activity. According to previous research, S. venosa contains several alkaloids, such as protoberberine stephanine cyclanoline and N-methylstepholidine, kamaline, (+)-N-carboxamidostepharine, (-)-O-methylstepharinosine, (-)-stepharinosine, aporphine (-)-O-acethylsukhodiamine and oxostephanosine. The chemical and biological investigations of this plant are interesting to bioassay-guided fractionation, particularly Antiproliferative effects via the induction of cell cycle arrest in mammalian cancer cell lines. Approach: The research was carried out to extract, isolate, purify and elucidate structure of the active compound from the tuber S. venosa. Most of the solvent extracts and isolated compound were evaluated with kinds of mammalian cancer cell lines for investigation on antiproliferative effects. Results: Four alkaloids, tetrahydropalmatine (1), crebanine (2) O-methylbulbocapnine (3) and N-methyltetrahydropalmatine (4) were isolated from the tuber of S. venosa. Charaterization of the compounds were carried out by extensive NMR studies using COSY, HMQC, HMBC and DEPT in addition to other spectroscopic methods. These compounds (1, 2 and 3) were showed evidence of the anticancer activities for cell proliferation inhibition in K562, K562/Adr, GLC4 and GLC4/Adr cell lines due to G0/G1 obstruction by compound 2 and 3 and negligible S phase arrest by compound 1. Conclusion: The result showed slightly increase in S phase by the effect of compound 1, beside the G0/G1 phase was blocked by compound 2 and 3.

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Keywords

  • Stephania venosa
  • Menispermaceae
  • palmatine
  • aporphine
  • crebanine and bulbocapnine alkaloids
  • cell cycle arrest
  • cytotoxicity