Research Article Open Access

Determination of the Presence of Huanglongbing in Seeds and Movement of the Pathogen in Citrus reticulata

Hajivand Shokrollah1, Thohirah Lee Abdullah2, Kamaruzaman Sijam1 and Siti Nor Akmar Abdullah2
  • 1 ,
  • 2 , Afganistan
American Journal of Applied Sciences
Volume 6 No. 6, 2009, 1180-1185

DOI: https://doi.org/10.3844/ajassp.2009.1180.1185

Submitted On: 13 March 2009 Published On: 30 June 2009

How to Cite: Shokrollah, H., Abdullah, T. L., Sijam, K. & Abdullah, S. N. A. (2009). Determination of the Presence of Huanglongbing in Seeds and Movement of the Pathogen in Citrus reticulata. American Journal of Applied Sciences, 6(6), 1180-1185. https://doi.org/10.3844/ajassp.2009.1180.1185

Abstract

Problem statement: Huanglongbing (HLB) also known as citrus greening disease is a fastidious phloem-inhabiting bacterium in the genus Candidatus Liberibacter. Using universal primers, the 16S ribosomal DNA sequence of three strains of the bacterium were obtained by PCR. However there is very little information on seed transmission and HLB pathogen movement to find a way for control or reduce the severity of HLB on the field. The study was conducted to detect HLB pathogen in seeds of Citrus, to determine pathogen movement in citrus seedling after infection and to detect the HLB pathogen in citrus roots. Approach: Seeds of Citrus reticulata cv. Limau Madu were collected from infected orchard and were germinated in screenhouse condition. The seeds of Citrus reticlata cv. L. Madu were planted in screenhouse too for HLB pathogen movement and HLB detection in roots. The seedlings were inoculated using infected grafting methods. Results: HLB was not amplified in new seedlings after germination. HLB moved slowly reaching up to 1.5 cm after 2 weeks, 1.5-4.5 cm after eight weeks and detected on 4.5-9 cm after 14 weeks below the grafting area. HLB was also detected up to 9-15 cm after 16 weeks, 15-24 cm after twenty weeks, 24-28.5 cm after 22 weeks and 28.5-30 cm after 24 weeks below the grafting area. Conclusion: Base on conventional PCR test, HLB disease in citrus is not seed borne and it can reach to the roots 26 weeks after inoculation.

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Keywords

  • HLB
  • seed borne
  • pathogen movement
  • greening disease
  • citrus