Research Article Open Access

Separation of Coenzyme Q10 in Palm Oil by Supercritical Fluid Chromatography

Ng Mei Han1, Choo Yuen May1, Ma Ah Ngan1, Chuah Cheng Hock1 and Mohd Ali Hashim1
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American Journal of Applied Sciences
Volume 3 No. 7, 2006, 1929-1932

DOI: https://doi.org/10.3844/ajassp.2006.1929.1932

Submitted On: 9 January 2006 Published On: 31 July 2006

How to Cite: Han, N. M., May, C. Y., Ngan, M. A., Hock, C. C. & Hashim, M. A. (2006). Separation of Coenzyme Q10 in Palm Oil by Supercritical Fluid Chromatography. American Journal of Applied Sciences, 3(7), 1929-1932. https://doi.org/10.3844/ajassp.2006.1929.1932

Abstract

Palm oil is known to host a variety of phytonutrients; some having antioxidant property such as the carotenes and vitamin E. These antioxidants are also present in the oil recovered from the palm-pressed fibre. Study was carried out to investigate the presence of coenzyme Q10, yet another non-glyceride compound which possesses antioxidant property in crude palm oil (CPO) and palm fibre oil. Separation of coenzyme Q10 in CPO and palm fibre oil was carried out using supercritical fluid chromatography with ultra violet detection. 1H and 13C NMR were used for its characterisation. Calibration with authentic standard shows that there are 10-80 ppm coenzyme Q10 in CPO while its concentration in palm fibre oil is 1000-1500 ppm.

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Keywords

  • Coenzyme Q10
  • crude palm oil
  • NMR
  • palm pressed fibre
  • supercritical fluid chromatography