Research Article Open Access

Production of Cellulase from Oil Palm Biomass as Substrate by Solid State Bioconversion

Md. Z. Alam1, Nurdina Muhammad1 and Mohd E. Mahmat1
  • 1 ,
American Journal of Applied Sciences
Volume 2 No. 2, 2005, 569-572

DOI: https://doi.org/10.3844/ajassp.2005.569.572

Submitted On: 15 February 2006 Published On: 28 February 2005

How to Cite: Alam, M. Z., Muhammad, N. & Mahmat, M. E. (2005). Production of Cellulase from Oil Palm Biomass as Substrate by Solid State Bioconversion. American Journal of Applied Sciences, 2(2), 569-572. https://doi.org/10.3844/ajassp.2005.569.572

Abstract

Solid state bioconversion (SSB) of lignocellulosic material oil palm biomass (OPB) generated from palm oil industries as waste was conducted by evaluating the enzyme production through filamentous fungus in lab-scale experiment. OPB in the form of empty fruit bunches (EFB) was used as the solid substrate and treated with the fungus Trichoderma harzianum to produce cellulase. The results presented in this study revealed that the higher cellulase activity of 0.0413 unit was achieved at the day 3 of fermentation. While the optimum study indicated the enzyme production of 0.0433 unit with moisture content of 50%, 0.0413 unit with 5% v/w of inoculum size and 0.0413 unit with co-substrate concentration of 2% (w/w) at days 9, 9 and 12 of fungal treatment, respectively. The parameters glucosamine and reducing sugar were observed to evaluate the growth and substrate utilization in the experiment.

  • 1,399 Views
  • 2,380 Downloads
  • 28 Citations

Download

Keywords

  • Cellulase
  • Oil Plam Biomass
  • Solid State Bioconversion
  • Trichoderma harzianum