Application of Human Adipose Stem Cell (hADSC) in Collagen Gel on Rat Deep Dermal Burn
- 1 Master Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Indonesia
- 2 Departemen of Biology of Medicine, Faculty of Medicine, Universitas Indonesia, Indonesia
- 3 Department of Histology, Faculty of Medicine, Universitas Indonesia, Indonesia
Abstract
Human adipose derived stem cells (hADSC) is expected to be an efficient solution for healing deep-dermal burns that help in wound closure. This study used 20 male Sprague dawley rats. Each rat received three wounds with different treatments (control, hADSC in collagen gel and collagen gel). Wound closure was observed at day 7th, 14th, 21th and 28th (sacrificed day) macroscopically, wound areas were measured and collagen densities were evaluated. Reduction of wound area showed significant difference between hADSC in collagen gel group and control group (p 0.001) and between hADSC in collagen gel group and collagen gel group. The hADSC in collagen gel group had a higher collagen density compared to control and collagen gel group. Application of hADSC in bovine collagen gel in rat deep-dermal burn models provided faster and better wound healing compared with control and bovine collagen gel group.
DOI: https://doi.org/10.3844/ojbsci.2019.192.202
Copyright: © 2019 Helsy Junaidi, Puji Sari, Dwi Pratami Septiara, Jeanne A. Pawitan and Luluk Yunaini. This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Burns
- hADSC
- PCR
- Bovine Collagen Gel