OnLine Journal of Biological Sciences

Morphogenesis in Tissue Culture of Local Kazakh Cotton Varieties

Bishimbayeva Nazira, Yertayeva Bakhyt, Amirova Aigul and Rakhimbayev Izbasar

DOI : 10.3844/ojbsci.2017.178.186

OnLine Journal of Biological Sciences

Volume 17, Issue 3

Pages 178-186

Abstract

Callus was initiated from hypocotyl and cotyledon explants of seven of commercial Kazakh cotton cultivars on MS medium supplemented with 0,1 mg L-1 2,4-D and 0,5 mg L-1 kinetin. The percentage of callus formation ranged from 64,0 to 91,0% for cotyledons and from 72 to 90% for hypocotyls. Study of morphological heterogeneity peculiarities of metamorphosis and histological structure of calli of cotton allowed to identifying seven tissues types and selecting the morphologically stable and perspective callus type for embryogenesis. Significant differences between the calli types of cotton were found by histological and morphological comparative analysis.

Copyright

© 2017 Bishimbayeva Nazira, Yertayeva Bakhyt, Amirova Aigul and Rakhimbayev Izbasar. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.