A Kit for Multiplexed Detection of Antibodies to Agents of Hemotransmissible Infections
Alexander Poltavchenko, Anna Ersh, Pavel Filatov, Oleg Nechitaylo, Yulia Krupnitzkaya and Vadim Gureev
DOI : 10.3844/ojbsci.2016.137.144
OnLine Journal of Biological Sciences
Volume 16, Issue 3
The paper presents the results of laboratory trials of method using complex initial testing of antibodies to six agents of hemotransmissible infections: HIV, hepatitis B and C viruses, cytomegalovirus, Treponema pallidum and Toxoplasma gondii. The method is based on multiplexed dot immunoassay using plane protein arrays (immunochips) with the use of colloidal gold conjugates and silver development. We describe composition of the kit enabling us to conduct dot assay at room temperature within 70 min. It was shown that the results of multiplexed analysis correlate well with data obtained using commercially available monospecific kits for ELISA. Furthermore, multiplexed dot assay is faster and more efficient in execution as compared to ELISA and can be carried out in field conditions.
© 2016 Alexander Poltavchenko, Anna Ersh, Pavel Filatov, Oleg Nechitaylo, Yulia Krupnitzkaya and Vadim Gureev. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.