A Kit for Multiplexed Detection of Antibodies to Agents of Hemotransmissible Infections
- 1 Laboratory of Immunochemical Methods in Diagnostics, State Research Center of Virology and Biotechnology “Vector”, Koltsovo, Russia
Abstract
The paper presents the results of laboratory trials of method using complex initial testing of antibodies to six agents of hemotransmissible infections: HIV, hepatitis B and C viruses, cytomegalovirus, Treponema pallidum and Toxoplasma gondii. The method is based on multiplexed dot immunoassay using plane protein arrays (immunochips) with the use of colloidal gold conjugates and silver development. We describe composition of the kit enabling us to conduct dot assay at room temperature within 70 min. It was shown that the results of multiplexed analysis correlate well with data obtained using commercially available monospecific kits for ELISA. Furthermore, multiplexed dot assay is faster and more efficient in execution as compared to ELISA and can be carried out in field conditions.
DOI: https://doi.org/10.3844/ojbsci.2016.137.144
Copyright: © 2016 Alexander Poltavchenko, Anna Ersh, Pavel Filatov, Oleg Nechitaylo, Yulia Krupnitzkaya and Vadim Gureev. This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Antibodies
- Multiplexed Dot Assay
- Hemotransmissible Infections
- Complex Initial Test
- ELISA