Proposed Insights in the Pharmaco-Dynamic Theory of Ascorbate Pro-Oxidant Activity
Kheir Eddine Kerboua, Fatma Haiba, Fatima Bendjemia, Mimouna Kerrache and Amina Boumediene
DOI : 10.3844/ojbsci.2016.1.9
OnLine Journal of Biological Sciences
Volume 16, Issue 1
Ascorbate pro-drug theory promising possible effective and nontoxic treatment is intensively examined and centered on the importance of both extracellular and intra-cellular effects of ascorbate. In this study, we sought to determine the in vitro cell responsiveness in a plasma free milieu with or without an extracellular catalyst and/or hydrogen peroxide. This project brought to light how ascorbic acid would react to in a Red Blood Cell (RBC) suspension by using the two anti-oxidant pathways. We demonstrated that while ascorbate doesn’t require an extracellular metal catalyst to induce cell damage via the lipid peroxidation pathway, the protein-centered metal pathway could be enhanced by a divalent cation, hydrogen peroxide or both. The current lack of understanding of ascorbate potential mechanism excites us to propose some insights for the vitamine C pharmacologic dose pro-oxidant activity.
© 2016 Kheir Eddine Kerboua, Fatma Haiba, Fatima Bendjemia, Mimouna Kerrache and Amina Boumediene. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.