Subcellular Localization Studies of Three Phenylalanine Ammonia-Lyases and Cinnamate 4-Hydroxylase from Scutellaria Baicalensis Using GFP Fusion Proteins | Science Publications

OnLine Journal of Biological Sciences

Subcellular Localization Studies of Three Phenylalanine Ammonia-Lyases and Cinnamate 4-Hydroxylase from Scutellaria Baicalensis Using GFP Fusion Proteins

Nam Il Park, Hui Xu, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi and Sang Un Park

DOI : 10.3844/ojbsci.2015.70.73

OnLine Journal of Biological Sciences

Volume 15, Issue 2

Pages 70-73

Abstract

The localization of three phenylalanine ammonia-lyases (PAL1, -2 and -3) and Cinnamate 4-Hydroxlase (C4H) of Scutellaria baicalensis was examined in onion epidermal cells. These genes encode key enzymes in the phenylpropanoid pathway for the synthesis of flavones. In our previous research, we isolated coding DNA for these genes from S. baicalensis, a medicinal herb rich in flavones with biological and pharmacological properties. We observed that SbPAL2, SbPAL3 and SbC4H proteins localize to the endoplasmic reticulum; however, SbPAL1 was a cytosolic protein. Unlike SbPAL2 and SbPAL3, SbPAL1 may be expected to have a different function in the flavone biosynthetic pathway.

Copyright

© 2015 Nam Il Park, Hui Xu, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi and Sang Un Park. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.