OnLine Journal of Biological Sciences

GRAPHENE OXIDE AS ANTIMICROBIAL AGAINST TWO GRAM-POSITIVE AND TWO GRAM-NEGATIVE BACTERIA IN ADDITION TO ONE FUNGUS

Roda F. Al-Thani, Noorunnisa Khanam Patan and Mariam A. Al-Maadeed

DOI : 10.3844/ojbsci.2014.230.239

OnLine Journal of Biological Sciences

Volume 14, Issue 3

Pages 230-239

Abstract

Graphene based materials have wide potential applications in biology, biomedical, agriculture environmental and biotechnology. Graphene Oxide (GO) is one of those materials and has a promising substance as antimicrobial agents. GO in this study was prepared by a modified Hummers method and was characterized by different techniques for confirmation of formation of GO. To study the antimicrobial activities of GO, it was tested against these microorganisms, one eukaryotic fungus (Candida albicans, C. albicans) two Gram negative bacteria (Escherichia coli (E. coli) ATCC 41570 and Pseudomonas aeruginosa (P. aeruginosa) ATCC 25619) and two Gram positive bacteria (Streptococcus faecalis (S. faecalis) ATCC 19433 and Staphylococcus aureus (S. aureus) ATCC 11632). Anti-microbial activity of GO was detected by spectrophotometer as indirect method to measure the growth and viable cell count as direct method. Readings were taken at successive incubated times. Results revealed that GO has antibacterial and anti-fungal activity against microorganisms used in this study. In conculosion the developed GO exhibit excellent antimicrobial property and GO affects more on Gram positive bacteria than Gram negative bacteria and fungi.

Copyright

© 2014 Roda F. Al-Thani, Noorunnisa Khanam Patan and Mariam A. Al-Maadeed. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.