Research Article Open Access

SOME FEATURES OF HYDROLYSIS OF THE HYBRID B-Z-FORM DNA BY SERRATIA MARCESCENS NUCLEASE

Maria Filimonova1, Valentina Gubskaya1 and Il’dus Nuretdinov2
  • 1 Kazan Federal University, Russia
  • 2 Kazan Scientific Center of Russian Academy of Sciences, Russia

Abstract

Highly polymerized herring testis DNA of the random nucleotide sequence was used as a model of natural substrate to study some features of hydrolysis of the hybrid B-Z form with Serratia marcescens nuclease. The hybrid B-Z-form was formed upon addition of 1.15 M MgSO4 and 0.421 mM Co(NH3)6Cl3. The DNA transition from the right handed B-form to the hybrid B-Z-form caused a decrease in Vmax of DNA cleavage with the nuclease. The diminishing Vmax was consistent with diminishing values of Km and Kcat. The binding of Mg2+ or Co(NH3)63+ to highly polymerized DNA caused correspondingly about 80-or 7-fold decrease in Km and more than 1600 or 600 decrease in Kcat compared with that of Mg-DNA complex of B-form.

OnLine Journal of Biological Sciences
Volume 14 No. 3, 2014, 181-187

DOI: https://doi.org/10.3844/ojbsci.2014.181.187

Submitted On: 27 June 2014 Published On: 25 July 2014

How to Cite: Filimonova, M., Gubskaya, V. & Nuretdinov, I. (2014). SOME FEATURES OF HYDROLYSIS OF THE HYBRID B-Z-FORM DNA BY SERRATIA MARCESCENS NUCLEASE. OnLine Journal of Biological Sciences, 14(3), 181-187. https://doi.org/10.3844/ojbsci.2014.181.187

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Keywords

  • Hybrid B-Z-form DNA
  • Serratia Marcescens Nuclease
  • Sma Nuc
  • Mg2+
  • Co(NH3)63+