SOME FEATURES OF HYDROLYSIS OF THE HYBRID B-Z-FORM DNA BY SERRATIA MARCESCENS NUCLEASE
Maria Filimonova, Valentina Gubskaya and Il’dus Nuretdinov
DOI : 10.3844/ojbsci.2014.181.187
OnLine Journal of Biological Sciences
Volume 14, Issue 3
Highly polymerized herring testis DNA of the random nucleotide sequence was used as a model of natural substrate to study some features of hydrolysis of the hybrid B-Z form with Serratia marcescens nuclease. The hybrid B-Z-form was formed upon addition of 1.15 M MgSO4 and 0.421 mM Co(NH3)6Cl3. The DNA transition from the right handed B-form to the hybrid B-Z-form caused a decrease in Vmax of DNA cleavage with the nuclease. The diminishing Vmax was consistent with diminishing values of Km and Kcat. The binding of Mg2+ or Co(NH3)63+ to highly polymerized DNA caused correspondingly about 80-or 7-fold decrease in Km and more than 1600 or 600 decrease in Kcat compared with that of Mg-DNA complex of B-form.
© 2014 Maria Filimonova, Valentina Gubskaya and Il’dus Nuretdinov. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.