INDUCED PLURIPOTENT STEM CELLS AND THEIR IMPLICATION FOR BIOMEDICAL SCIENCES
Maria Csobonyeiova, Stefan Polak and Lubos Danisovic
DOI : 10.3844/ojbsci.2013.106.114
OnLine Journal of Biological Sciences
Volume 13, Issue 4
The groundbreaking discovery, that somatic mammalian cells can be epigenetically reprogrammed to a pluripotent state through the exogenous expression of the transcription factors Oct4, Sox2, Klf4 and c-myc, has yielded a new cell type for potential application in regenerative medicine, the induced Pluripotent Stem Cells (iPSCs). Since the first demonstration of creating iPSCs in 2006 great efforts have been made into improving iPS cell generation methods and understanding the reprogramming mechanism as well as the nature of iPSCs. The iPSCs technique makes it possible to produce patient-specific pluripotent stem cells for transplantation therapy without immune rejection. However, some restriction still remain, including viral vector integration into the genome, the existence of exogenous oncogenic factors and low induction efficiency. In this review we discuss recent advances in methods of generating safer iPSCs lines and their possible use for biomedical applications.
© 2013 Maria Csobonyeiova, Stefan Polak and Lubos Danisovic. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.