The Effect of Salivary Gland Extract of Lucillia Sericata Maggots on the Human Dermal Fibroblasts Proliferation
Simona Polakovicova, Stefan Polak, Marcela Kuniakova, Marek Cambal, Juraj Majtan, Peter Takac, Milan Kozanek and Lubos Danisovic
DOI : 10.3844/ojbsci.2011.96.100
OnLine Journal of Biological Sciences
Volume 11, Issue 3
Problem statement: The aim of study was to investigate the effect of Salivary Gland Extract (SGE) of Lucillia sericata maggots on Human Neonatal Fibroblasts (B-HNF-1) proliferation and migration within collagen/hyaluronan membrane. Chronic, non-healing wounds are major health care problem worldwide. Nowadays, Maggot Debridement Therapy (MDT) has attracted much attention due to its successfull application in a treatment of chronic wounds where conventional treatment has failed. Maggot Excretion/Secretion (ES) contains specific proteinases which are positivelly involved in the remodeling of Extracelallar Matrix (ECM) components. Approach: Immunohistochemical methods using primary monoclonal antibody against marker vimentin was employed for the visualization of fibroblasts cultured within collagen hyaluronan membrane. Results: We found that maggot SGE at concentration of 2.4 and 4.8 gL mL-1 significantly induced the migration of fibroblasts into the membrane after 5 and 10 days of cultivation. On the other hand, SGE at concentration of 7.2 gL mL-1 reduced the migration of fibroblasts. Conclusion: The obtained results suggest that medicinal maggots secretion may speed up the wound healing process through the stimulation of fibroblast migration. However, further detailed research is needed to fully elucidate the substance (s) responsible for fibrloblast stimulation and migration.
© 2011 Simona Polakovicova, Stefan Polak, Marcela Kuniakova, Marek Cambal, Juraj Majtan, Peter Takac, Milan Kozanek and Lubos Danisovic. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.