Using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction of Barbodes spp. in Thailand
Keeravit Petjul, Aphidech Sangdee and Bungorn Thaewnon-ngiw
DOI : 10.3844/ojbsci.2011.1.6
OnLine Journal of Biological Sciences
Volume 11, Issue 1
Problem statement: The genetic diversity of Barbodes species in Thailand is presently unavailable. The objectives of this study were to study external morphology and genetic diversity using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) of three Barbodes species; Barbodes altus, B. gonionotus and Barbodes sp. Approach: Two of twelve screened decanucleotide primers (OPA07 and UBC122), were selected for the genetic analysis of Barbodes genera. Results: Thirty-five reproducible and polymorphic fragments (250-2150 bp in length) were generated across the investigated species. The percentages of polymorphic band were 7.69, 3.85 and 6.25% for B. altus, B. gonionotus and Barbodes sp., respectively. The UPGMA indicated a similarity coefficient between B. gonionotus and Barbodes sp. up to 90%. The candidate species-specific marker of UBC122 primer was found in only Barbodes sp. Conclusion: This approach indicates that Barbodes sp. may be a variant species of B. gonionotus.
© 2011 Keeravit Petjul, Aphidech Sangdee and Bungorn Thaewnon-ngiw. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.