Susceptibility of Candida Species Isolated From HIV Infected and Newborn Candidaemia Patients to Amphotericin B
Saikat Basu, Debasrita Chakraborty and Satadal Das
DOI : 10.3844/ojbsci.2010.109.113
OnLine Journal of Biological Sciences
Volume 10, Issue 2
Problem statement: HIV infected and neonatal candidaemia is a serious condition where proper selection of the antifungal agent is the key factor in the prognosis of the disease. Thus in this study susceptibility of Candida species to Amphotericin B was explored in such patients. Approach: Forty different Candida isolates obtained from blood culture of such candidaemia cases were tested for Amphotericin B susceptibility by disc diffusion method and by microdilution method. Results: There was a good correlation between low MIC values and large inhibition zones among most isolates as expected, particularly with 24 h reading. Several outlier data were also observed during the study. After analysis it was found that 24 h microdilution method results were better for interpretation of susceptibility results. Conclusion: Microdilution method is the reliable method for susceptibility testing of Candida isolates from HIV infected and neonatal candidaemia patients to Amphotericin B.
© 2010 Saikat Basu, Debasrita Chakraborty and Satadal Das. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.