OnLine Journal of Biological Sciences

Characteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71

Farouk Al-Quadan, Hazem Akel and Rasha Natshi

DOI : 10.3844/ojbsci.2009.67.74

OnLine Journal of Biological Sciences

Volume 9, Issue 3

Pages 67-74

Abstract

Problem statement: This study reported the purification and characterization of a novel highly thermostable alkaline amylase from a newly isolated Bacillus strain HUTBS71. Approach: The enzyme was purified using ammonium sulfate precipitation, ion exchange and gel filtration chromatography. Results: Maximum amylase activity (72 U mL-1) was obtained at 100

Copyright

© 2009 Farouk Al-Quadan, Hazem Akel and Rasha Natshi. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.