Trace Metal Effect on Hydrogen Production Using C.acetobutylicum
Hisham Alshiyab, Mohd Sahaid Kalil, Aidil Abdul Hamid and Wan Mohtar Wan Yusoff
DOI : 10.3844/ojbsci.2008.1.9
OnLine Journal of Biological Sciences
Volume 8, Issue 1
This study was carried out to study the effect of trace metal addition for hydrogen production The results show that this bacterial need a selected nutrient with selected concentration. The optimum parameters for cultivation were at initial pH of 7.0 and temperature of 30Â°C. Trace metal addition to the new medium showed that only iron enhanced the H2 yield. The results showed that 25 mgLâ1 FeSO4â 7H2O enhanced the hydrogen yield from 391 mLgâ1 to 408 mLgâ1 glucose utilized with biomass concentration of 1.38 gLâ1. YP/X of 292 mLgâ1 Lâ1, YX/S of 0.276 and YH2/s of 0.0364, gave the maximum hydrogen productivity of 81.6 mL Lâ1 hâ1. Magnesium and calcium addition were better for growth and not for hydrogen production. Biomass concentration increases as trace metal increases in the fermentation medium. The highest biomass concentration was 1.65 gLâ1 at 1000 mgLâ1 FeSO4â 7H2O, 1.47 gLâ1 at 1000 mgLâ1 MgSO4â 7H2O and 1.73 gLâ1 at 500 mgLâ1 CaCl2â 2H2O, suggested that all trace metal are necessary for biomass production.
© 2008 Hisham Alshiyab, Mohd Sahaid Kalil, Aidil Abdul Hamid and Wan Mohtar Wan Yusoff. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.