OnLine Journal of Biological Sciences

Characterization of Bacterial Strains Isolated Through Microbial Profiling of Urine Samples

Poulomi Nandy, A. R. Thakur and S. Ray Chaudhuri

DOI : 10.3844/ojbsci.2007.44.51

OnLine Journal of Biological Sciences

Volume 7, Issue 1

Pages 44-51

Abstract

The present study was conducted to determine the microbial profile in urine samples. Differential and selective chromogenic culture media were used for the rapid detection, identification and enumeration of urinary tract pathogens namely, E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Proteus mirabilis. Urine samples of normal healthy individuals as well as patients with Urinary Tract Infection (UTI) were screened on hicrome agar plates. The cultivable bacteria present in urine were isolated based on chromogenic detection. Antibiotic sensitivity assay, morphological characterization and biochemical tests, namely protease, oxidase, catalase, lipase, DNase and lecithinase assay were performed with the 15 isolates obtained from urine samples. The molecular analyses of the isolates were done through partial sequencing of the 16SrDNA gene; six of them were found to be novel and submitted in GenBank under the accession numbers EF644491-96. Phylogenetic tree of the isolates were constructed by neighbour joining method.

Copyright

© 2007 Poulomi Nandy, A. R. Thakur and S. Ray Chaudhuri. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.