OnLine Journal of Biological Sciences

The Role of Sucrose in the Development of Oral Biofilm in a Simulated Mouth System

Wan Nordini Hasnor Wan Ismail, Fathilah Abdul Razak and Zubaidah Hj Abdul Rahim

DOI : 10.3844/ojbsci.2006.62.66

OnLine Journal of Biological Sciences

Volume 6, Issue 2

Pages 62-66

Abstract

The development of oral biofilm consisting of early settlers and late settlers in the presence and absence of sucrose using a simulated mouth model was investigated. The experiments were carried out by growing the early settlers’ biofilm consisting of Strep. mitis and Strep. sanguinis and late settlers’ biofilm of Strep. mutans in the simulated mouth system. Experiments using three different nutrient conditions (A, B and C) were carried out. In A, sterile saliva was used as the nutrient source in which the early settlers were suspended and pumped into the simulated mouth system and allowed to grow for 24 hrs. In B, 1% of BHI broth was added to the sterile saliva three times a day at 6 hrs interval to demonstrate the effect of glucose on the development of the biofilms. In C, the 1% BHI was supplemented with 1% sucrose in order to show the effect of glucose in the presence of sucrose on the development of the biofilm. The experiments were subsequently repeated with the late settlers. In all the experiments, A serves as the control where the nutrient source is minimal. The data collected were analyzed using Student t-test. From the results obtained, it was observed that both glucose and sucrose enhanced the development of early and late settlers’ biofilms. The growth of late settlers’ biofilm however, was found to be significantly higher in the presence of sucrose (93x106 CFU mL-1) compared to glucose (66x106 CFU mL-1) with a p value <0.05.

Copyright

© 2006 Wan Nordini Hasnor Wan Ismail, Fathilah Abdul Razak and Zubaidah Hj Abdul Rahim. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.