Research Article Open Access

Construction of a Glucose Biosensor by Immobilizing Glucose Oxidase within a Poly(o-phenylenediamine) Covered Screen-printed Electrode

Miao Yuqing1, Chen Jianrong1 and Wu Xiaohua1
  • 1 Zhejiang Normal University Jinhua 321004, China

Abstract

The glucose biosensors were prepared by the electropolymerization of the non-conductive polymer, Poly(o-phenylenediamine), onto a planar screen-printed electrode. A fabrication procedure was performed to decrease the waste of expensive enzyme. The amperometric glucose response was measured by the potensiostating of the prepared glucose biosensors at a potential of 0.3 V with ferrocene as mediator. Results show that the obtained biosensors have a linear range up to 25 mM glucose, fast response time (100s) and high sensitivities (16.6 nA/mM). Also, the effects of applied potential and sweeping number of Cyclic voltammograms for electropolymerization were systematically investigated and optimal values were recorded.

OnLine Journal of Biological Sciences
Volume 6 No. 1, 2006, 18-22

DOI: https://doi.org/10.3844/ojbsci.2006.18.22

Submitted On: 17 April 2006 Published On: 30 June 2006

How to Cite: Yuqing, M., Jianrong, C. & Xiaohua, W. (2006). Construction of a Glucose Biosensor by Immobilizing Glucose Oxidase within a Poly(o-phenylenediamine) Covered Screen-printed Electrode. OnLine Journal of Biological Sciences, 6(1), 18-22. https://doi.org/10.3844/ojbsci.2006.18.22

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Keywords

  • Poly(o-phenylenediamine)
  • non-conducting polymer
  • biosensor
  • glucose