OnLine Journal of Biological Sciences

 Association of High-Affinity FcεRI-β intron 2 for IgE Gene Polymorphism and Atopic Diseases in an Algerian Population

Dahbia Ines Dahmani, Ikhlass Haj Salem, Jamila Chakir, Karima Sifi, Sellami Abderrahim, Mounir Zhary Bachtarzi, Sara Malki, Laila Rouabah, Nore-Eddine Abadi, Mehdioui Hacene, Mohamed Bougrida and Mahmoud Rouabhia

OnLine Journal of Biological Sciences

Abstract

Atopy is characterized by the production of a high level of IgE in response to common allergens. We examined the RsaI-in2 polymorphism of the FcεRI-β gene and its association with atopic asthma and comorbidity. The study population included 77 atopic asthmatics and 77 non-atopic, non-allergic and non-asthmatic controls. Atopic asthma was confirmed by the skin prick test and by IgE and interleukin IL-4 levels. RsaI-in2 polymorphism was determined by RFLP-PCR using the RsaI enzyme. A significant correlation was found between genotype BB and sensitization to house dust mites in the asthmatic subjects (p = 0.000). Results also reveal significant allele sharing in the subjects affected by eczema (p = 0.000) and allergic rhinitis (p = 0.01) during childhood. Furthermore, the subjects with heterozygous AB and homozygous BB genotypes displayed significant IgE levels > 1000 UI mL-1. IL-4 levels were also > 1000 pmol/ml for RsaI-in2 polymorphism genotype BB. Our findings show a strong association between genotype BB and the incidence of eczema and allergic rhinitis in childhood as well as increased IgE and IL-4 levels.

Copyright

© 0000 Dahbia Ines Dahmani, Ikhlass Haj Salem, Jamila Chakir, Karima Sifi, Sellami Abderrahim, Mounir Zhary Bachtarzi, Sara Malki, Laila Rouabah, Nore-Eddine Abadi, Mehdioui Hacene, Mohamed Bougrida and Mahmoud Rouabhia. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.