American Journal of Biochemistry and Biotechnology

The Investigation of Organelle Genomes of Extra Nuclear Sunflower Mutants with Variegated Phenotype

Irina Dremuk, Nikolay Kozel, Mariya Logacheva, Oleg Gorbachenko, Kirill Azarin, Nikolay Markin, Alexander Usatov and Maxim Makarenko

American Journal of Biochemistry and Biotechnology

Abstract

The comparative analysis of complete chloroplast andmitochondrial DNA sequences of the original inbred line 3629 and three extranuclear mutant lines variegated-1, variegated-15, variegated-17,obtained by the N-nitroso-N-methylurea induced mutagenesis, was done. Thestudied mutant lines were presented two different phenotypes with diversechlorophyll content: Pale/green mutant line variegated-17 with 1.8%relative (to 3629 line) chlorophyll content in mutant leaf tissue andyellow/green lines variegated-1, variegated-15 with 6.3% and 4.3%relative chlorophyll content, respectively. Each line had unique variationsites in chloroplast DNA. Among chloroplast SNP there were determined 7nonsynonymous substitutions, which were located in psaA (variegated-1),petD, rpl36, ccsA (variegated-15), rps4, rpoA,rpoC1 (variegated-17). The SNP of variegated-17 line has resultedin the frameshift (rpoC1) andpremature stop codon formations (rpoA, rpoC1). The whole genomesequencing of mitochondrial DNA mutant lines revealed no differences ascompared with original line 3629. Summarizing current data and our previousresearch of variegated mutants we assume that pale/green variegated phenotypeis associated with significant disturbance of RNA polymerase subunits genes,and the lesions in photosynthetic genes lead to yellow/green mutant phenotype.

Copyright

© 2017 Irina Dremuk, Nikolay Kozel, Mariya Logacheva, Oleg Gorbachenko, Kirill Azarin, Nikolay Markin, Alexander Usatov and Maxim Makarenko. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.