A Computer Simulation Model for Automated Quantification of Luteinizing Hormone Secretion
Ayman Al-Nsour, Hamza A. Ali, Buthynna Al-Dulaimi and Wisam H. Al-Sewadi
DOI : 10.3844/jcssp.2009.419.426
Journal of Computer Science
Volume 5, Issue 6
Problem statement: An automated software package is developed for quantifying Luteinizing Hormone (LH) release from pituitary gonadotropes in response to short pulses of Gonadotropin-Releasing Hormone (GnRH). Approach: These computer programs were designed to accommodate the release mechanism that consists of three stages, namely; (1) Production of different concentrations of effectors (such as receptor-hormone binds, G protein interactions, inositol 1,4,5-trisphosphate IP3), (2) Release of Ca2+ from the endoplasmic reticulum ER and finally (3) Release of LH through pumping of Ca2+ in and out of the cytosol. Results: The programs were coded in C++ computer language and implemented on Pentium PC. The designed model was intended to help in explaining the characteristics of LH release in response to various duration and different concentrations of GnRH pulses in the presence and absence of external Ca2+. Conclusion: Features of the short-term responses were simulated and fairly understood using receptor binding, dimerization, interaction of the dimerized receptor with a G protein, production of effectors that open voltage sensitive channels in cell membrane and finally open the Ca2+ channels in the ER and the Ca2+-dependent release of LH. Also, prediction of the varying concentrations of Ca2+ effects and comparison with experimental data was made possible.
© 2009 Ayman Al-Nsour, Hamza A. Ali, Buthynna Al-Dulaimi and Wisam H. Al-Sewadi. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.