Current Research in Dentistry

The Effect of two Different Culture Media on the Proliferation of Dental Pulp Stem Cells: Bovine Fetal Serum and Platelet Lysate

Giuseppe Cicero, Veena Nanda and Irene Martini

DOI : 10.3844/crdsp.2015.13.17

Current Research in Dentistry

Volume 6, Issue 2

Pages 13-17

Abstract

Mesenchymal Stem Cells (MSCs) have been isolated from a variety of fetal and adult tissues and are considered an ideal source for cell-based therapy due to their unique properties such as multipotency and immunomodulatory functions. The aim of this work is to observe, analyze and investigate the morphology and proliferation of human dental pulp stem cells cultured in two different culture media: Fetal Bovine Serum (FBS) or platelet lysate. These cells would be cultured for a minimum period of 14 days to 3 months. The desired outcome is a pure culture of mesenchymal stem cells. With this present study we will determine which culture mediumpromotesthe most rapid formation of a pure culture of mesenchymal stem cells derived from Dental Pulp Stem Cells (DPSC).

Copyright

© 2015 Giuseppe Cicero, Veena Nanda and Irene Martini. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.