American Journal of Pharmacology and Toxicology

Assessment of Type 2 Anti-Diabetes on Bound Flavonoids of Barringtonia racemosa (L.) Spreng. Kernel in Glucose-Induced Diabetic Rats

Musri Musman, Emelda Audina, Fazlia Inda Rahmayani Ratu, Erlidawati Erlidawati and Safrida Safrida

DOI : 10.3844/ajptsp.2017.48.61

American Journal of Pharmacology and Toxicology

Volume 12, Issue 3

Pages 48-61


A study of type 2 anti-diabetes on bound flavonoids fraction from the kernel of Barringtonia racemosa (L.) Spreng. in glucose-induced diabetic rats was performed. This learning aimed to gather scientific information about the possibility to utilize kernel of B. racemosa as a raw material of antidiabetic drug. The antioxidant property of the bound flavonoids was determined by the DPPH scavenging method compared to the ascorbic acid. Assessment of type 2 anti-diabetes was conducted on glucose-induced diabetic Rattus norvegicus Wistar strain compared to metformin and the data were analyzed through one-way ANOVA software. Histopathological studies of the pancreas and kidney were made to get evidence of the β-cell performance and the renal tissue respectively. The DPPH testing at the wavelength of 517 nm showed the bound flavonoids and the ascorbic acid showed absorbance at 0.097 and 0.080 correlated with IC50 values of 7.51 and 6.50 ppm respectively. The results of one-way ANOVA indicated that the administration of bound flavonoids was significant (F(2,11) = 8.60, p = 0.008) to reduce blood glucose level in the tested rats. The diabetic rats treated with the extract experienced an antidiabetic effect equivalent to an antidiabetic effect of metformin. Histopathologic observations showed increasing of the granulated β-cell (F(3, 15) = 26.09, p<0.0001) and no renal tissue damage (F(3, 15) = 0.23, p = 0.873) in the tested rats. The conclusion raised from the data of this study revealed that the bound flavonoids from the kernel of B. racemosa (L.) Spreng. could be utilized as a drug source of type 2 anti-diabetes.


© 2017 Musri Musman, Emelda Audina, Fazlia Inda Rahmayani Ratu, Erlidawati Erlidawati and Safrida Safrida. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.