Biochemical Alterations Induced by Amylin in Wistar Rats
Mohamed Mohamed Soliman and Zein Shaban Ibrahim
DOI : 10.3844/ajptsp.2013.64.72
American Journal of Pharmacology and Toxicology
Volume 8, Issue 2
Amylin is a peptide hormone synthesized in β cells of pancreas together with insulin in response to glucose and nutrients. Amylin controls food intake and body weight but the exact mechanism is still unknown. This study aimed to examine the effects of amylin injection on hormones, metabolites and cytokines expression in rats. Wistar rats were injected amylin Intraperitoneally (IP) (10 µg kg-1 twice daily) for 7 days. Plasma and liver samples were collected for blood measurements and RT-PCR analysis. Amylin treatments induced significant decrease in body weight and food intake in time dependent manner. Moreover, amylin significantly increased insulin, leptin and lipase secretion. A decrease in plasma triglycerides, cholesterol and LPL and increase in HDL levels was recorded. There are upregulation in the expression of IL-1β and TNF-α in amylin treated rats. The results collectively indicate that amylin has anti-obesity like effects through regulation of proteins and obesity related gene expression.
© 2013 Mohamed Mohamed Soliman and Zein Shaban Ibrahim. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.