American Journal of Pharmacology and Toxicology

Involvement of Bone Marrow Megakaryocytes in the Formation of the Bone Marrow-Blood Barrier in Transgenic Mice

Pawel Laskowski, Karol Ostrowski, Beata Klim, Katarzyna Kitlas, Dorota Lemancewicz, Maria M. Winnicka and Janusz B. Dzieciol

DOI : 10.3844/ajptsp.2013.120.127

American Journal of Pharmacology and Toxicology

Volume 8, 2013

Pages 120-127

Abstract

Mature cell release takes place through the hypothetical barrier- the bone marrow-blood barrier. It consists of blood vessels and cells that surround them. Such cells are, among others, megakaryocytes. They are involved in the release of platelets or other cellular components of blood. The amount and the development of megakaryocytes and thus the maturity of barrier depends on many factors. One of them is IL-6, which gene is located on the short arm of chromosome 7 (7p15-p21). The study was performed on wild-type mice (C57B4/6J) and on mice lacking the gene encoding IL-6 (IL-6 KO-C57B4/6J IL-6tm1 Kopf-/-). Bone marrow of all animals in both groups has been collected, fixed and stained. Megakaryocytes were identified in each microscopic specimen using immunohistochemical reaction with the CD61 antibody. Then all specimens were subjected to histomorphometric analysis. Statistically significant differences in the total number and size of magakariocytes was noted, which affects bone marrow-blood barrier functions. This could be used in the conventional treatment of cancer, respiratory diseases and coagulation disorders and in the treatment using nanoparticles.

Copyright

© 2013 Pawel Laskowski, Karol Ostrowski, Beata Klim, Katarzyna Kitlas, Dorota Lemancewicz, Maria M. Winnicka and Janusz B. Dzieciol. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.