American Journal of Pharmacology and Toxicology

Influence of a Low-Level Semiconductor Gallium Arsenate Laser in Experimental Envenomation Induced by Bothrops atrox Snake Venom

Elziliam Aranha De Sousa, José Adolfo Homobono Machado Bittencourt, Nayana Keyla Seabra De Oliveira, Shayanne Vanessa Correia Henriques, Leide Caroline Dos Santos Picanço, Camila Pena Lobato, José Renato Ribeiro, Washington Luiz Assunção Pereira, José Carlos Tavares Carvalho and Jocivânia Oliveira Da Silva

DOI : 10.3844/ajptsp.2012.141.148

American Journal of Pharmacology and Toxicology

Volume 7, Issue 4

Pages 141-148

Abstract

Bothrops atrox is responsible for the majority of snakebite accidents in Brazilian Amazon and its venom can cause prominent local tissue damage. Experimental groups consisted of five male mice, each administered either B. atrox Venom (VB), B. atrox Venom + Antivenom (VAV), B. atrox Venom + Laser (VL), B. atrox Venom + Antivenom + Laser (VAVL), or Sterile Saline Solution (SSS) alone. Paw oedema was induced by intradermal administration of 0.05 mg kg-1 of B. atrox venom and was expressed in mm of directly induced oedema. Mice were subcutaneously injected with 0.10 mg kg-1 of venom for evaluation nociceptive activity and the time (in seconds) spent in licking and biting responses of the injected paw were taken as an indicator of pain response. Inflammatory infiltration was determined by counting the number of leukocytes present in the gastrocnemius muscle after venom injection (0.10 mg kg-1). Myotoxicity was studied by determining the plasmatic rise of creatine kinase activity after venom injection (0.20 mg kg-1). For histological examination of myonecrosis, venom (0.10 mg kg-1) was administered intramuscularly. The site of venom injection was irradiated by GaAs laser and some animals received antivenom intraperitoneally. GaAs laser irradiation administered in conjunction with antivenom, reduced pain, oedema, inflammation and myonecrosis induced by B. atrox venom in mice. The combined antivenom and GaAs laser treatment was more effective than separately treatments. The results suggest that laser therapy may reduce the local effects induced by B. atrox venom when associated with antivenom.

Copyright

© 2012 Elziliam Aranha De Sousa, José Adolfo Homobono Machado Bittencourt, Nayana Keyla Seabra De Oliveira, Shayanne Vanessa Correia Henriques, Leide Caroline Dos Santos Picanço, Camila Pena Lobato, José Renato Ribeiro, Washington Luiz Assunção Pereira, José Carlos Tavares Carvalho and Jocivânia Oliveira Da Silva. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.