American Journal of Pharmacology and Toxicology

Characterization of Epoxidized and Non-Epoxidized Fatty Diethanolamides by High Performance Liquid Chromatography and Gas Chromatography

Choy Sin Lee and Song Cang Lee

DOI : 10.3844/ajptsp.2010.133.138

American Journal of Pharmacology and Toxicology

Volume 5, Issue 3

Pages 133-138

Abstract

Problem statement: This study was carried out for the characterization of epoxidized and non-epoxidized fatty diethanolamides by High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC). Approach: The fatty diethanolamides were synthesized by reacting 40% (w/w) of Epoxidized Palm Olein (EPOo) with 60% (w/w) Refined Bleached Deodorized Plam Kernel Olein (RBDPKOo) with Diethanolamine (DEA) at 110°C for 5 h. Results: The identities of the peaks were established by secondary standards that were derived from pure methyl esters. HPLC separated major non-epoxidized diethanolamide compounds, namely C10:0, C12:0, C14:0, C16:O, C18:1 and C18:0 and the molecular weights of the compounds were dentified by Liquid Chromatography-Mass Spectrometry (LC-MS). Conclusion: GC provided a more complete separation for the non-epoxidized diethanolamides, epoxidized diethanolamides, glycerol, Diethanolamine (DEA) and some minor compounds from the self-condensation of DEA and diethanolamides. Gas Chromatography-Mass Spectrometry (GC-MS) elucidated the chemical structure of the epoxidized diethanolamides.

Copyright

© 2010 Choy Sin Lee and Song Cang Lee. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.