Research Article Open Access

Studies On Bioemulsifier Production by Bacillus licheniformis PTCC 1595

Gholamreza D. Noudeh1, Mohammad H. Moshafi1, Payam Khazaeli1 and Farideh Akef2
  • 1 School of Pharmacy, Iran
  • 2 Kerman University of Medical Sciences, Iran

Abstract

Surfactants are amphipathic molecules which reduce surface and interfacial tensions and widely used in pharmaceutical, cosmetic, food and petroleum industries. Biosurfactants are the structurally diverse group of surface-active molecules synthesized by microorganisms. There are several advantages for biosurfactants in contrast with chemical surfactants, such as lower toxicity; higher biodegradability; better environmental compatibility; higher foaming; high selectivity and specific activity at extreme temperatures, pH and the ability to be synthesized from renewable feed-stock. In the present study, the production of bioemulsifer by Bacillus licheniformis PTCC 1595 has been studied. B. licheniformis was grown in the nutrient broth medium and bioemulsifer production was evaluated every 24 h by surface tension and emulsification index (E24). Then B. licheniformis PTCC 1595 was grown in nutrient broth with different conditions in order to get maximum production of bioemulsifer. The best culture medium was found to be nutrient broth medium supplemented with starch, Fe2+, Mn2+ and olive oil. After growing the bacteria, the microbial biomass was removed from the supernatant by acidic precipitation method. Its amphipathic structure was established by biochemical and spectroscopy methods and it was confirmed lipopeptide structure.

American Journal of Pharmacology and Toxicology
Volume 2 No. 4, 2007, 164-169

DOI: https://doi.org/10.3844/ajptsp.2007.164.169

Submitted On: 29 July 2007 Published On: 31 December 2007

How to Cite: Noudeh, G. D., Moshafi, M. H., Khazaeli, P. & Akef, F. (2007). Studies On Bioemulsifier Production by Bacillus licheniformis PTCC 1595. American Journal of Pharmacology and Toxicology, 2(4), 164-169. https://doi.org/10.3844/ajptsp.2007.164.169

  • 3,228 Views
  • 2,987 Downloads
  • 0 Citations

Download

Keywords

  • Bacillus licheniformis
  • bioemulsifer
  • surface tension
  • emulsification index