Cardiovascular Effect of Capapris spinosa Aqueous Extract. Part VI: in vitro Vasorelaxant Effect
N. A. Zeggwagh, J. B. Michel and M. Eddouks
DOI : 10.3844/ajptsp.2007.135.139
American Journal of Pharmacology and Toxicology
Volume 2, Issue 3
The aim of this study was to investigate the in vitro vasorelaxanteffect of Capparis spinosa aqueous extract (CSAE) at a dose of 10 mg mL-1. Aortic rings isolated from normal wistar rats were used. The addition of CSAE during the plateau phase of contraction, induced by noradrenaline and KCl, produced a rapid relaxation. Incubation of aortic ring with CSAE during 30min shifted the noradrenaline induced dose response curve (p<0.001) and the maximum response (p<0.001) was attenuated indicating that antagonist effect of the Î±1 adrenoreceptors was non-competitive. However, endothelium remove significantly reduced the vasorelaxant effect of CSAE (p<0.01). Furthermore, nitric oxide inhibition reduced the vasorelaxant effect of CSAE. However, cyclo-oxygenase inhibition did not affect the vasorelaxant effect of CSAE (p<0.05). Inhibition of L-type voltage dependent Ca2+ channels did not reduce the observed CSAE vasorelaxant effect (p<0.05). We conclude that CSAE at a dose of 10 mg ml-1 possess in vitro vasorelaxant effect which may by mediated via an Î±1 adrenoreceptors antagonism and/or modulation of nitric oxide synthesis.
© 2007 N. A. Zeggwagh, J. B. Michel and M. Eddouks. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.