Optimization of Cultural Conditions for Production of Exopolysaccaride by Halomonas marina HMA 103 under Batch-Culture
Arundhati Pal, Amrita Biswas, Sriya Chatterjee and A. K. Paul
DOI : 10.3844/ajmsp.2015.31.39
Current Research in Microbiology
Volume 6, 2015
A moderately halophilic bacterium, Halomonas marina HMA 103 (MTCC 8968) accumulating intracellular polyesters also produced significant amount of exopolysaccharides (EPS) under batch-culture. Production of EPS by the bacterium was more or less parallel with the growth in modified basal synthetic medium. During growth, it produced 0.46 g/L of EPS after 50 h of incubation and was accompanied by the complete utilization of glucose from the medium. EPS production was maximum in 2% (w/v) glucose followed by sucrose and maltose and was positively influenced by yeast extract, beef extract and peptone. Optimum concentrations of sulfate and phosphate for EPS production were 2.4% (w/v) and 0.03% (w/v) respectively. High concentration (10% w/v) of NaCl stimulated both growth and EPS production by H. marina HMA 103. Characteristically, this EPS is a heteropolymer of glucose and mannose and the Fourier Transform Infrared (FTIR) spectrum showed distinct absorption peaks at 3424.2, 1638.7, 1122.9 and 622.3 cm-1 indicating the presence of free hydroxyl, phenyl or carbonyl groups of carbohydrate. Further, the detailed physico-chemical analysis will indicate the significance and potential importance of this EPS in biotechnology.
© 2015 Arundhati Pal, Amrita Biswas, Sriya Chatterjee and A. K. Paul. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.