American Journal of Immunology

Functionalization Strategies for Antibodies: Lessons Learned

Christian Schmidt, Martin Geyer, Jörg Bohrisch, Verena Jentzen, Alexander Gorczyza, Karim Khalil, Marina Volkert, Mark A. Brown, Saadettin Sel and Joachim Storsberg

DOI : 10.3844/ajisp.2017.144.154

American Journal of Immunology

Volume 13, Issue 2

Pages 144-154

Abstract

Precious little is known about useful functionalization strategies of antibodies that will not impair either reactivity or specificity of this valuable reagent. We used an experimental approach to demonstrate our own experience with published protocols. The conjugates obtained were then tested with regard to their performance against commercially available detection kits. Our results are then viewed in light of published precedence to highlight areas where future effort is needed to refine such versatile tools.

Copyright

© 2017 Christian Schmidt, Martin Geyer, Jörg Bohrisch, Verena Jentzen, Alexander Gorczyza, Karim Khalil, Marina Volkert, Mark A. Brown, Saadettin Sel and Joachim Storsberg. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.