American Journal of Immunology

Nitric Oxide Donors with Therapeutic Strategic in Experimental Schistossomiasis Mansoni

Wander Rogério Pavanelli, Jean Jerley Nogueira da Silva, Carolina Panis, Thiago Mattar Cunha, Francisco José de Abreu Oliveira, Maria Claudia Noronha Dutra de Menezes, Ivete Conchon Costa, Graciele da Silva Thomé, Francisco Ordelei Nascimento da Silva, Eduardo Henrique Silva de Sousa, Luiz Gonzaga de França Lopes, Rubens Cecchini, Fernando de Queiroz Cunha, Eiko Nakagawa Itano and Maria Angélica EharaWatanabe

DOI : 10.3844/ajisp.2014.225.239

American Journal of Immunology

Volume 10, Issue 4

Pages 225-239

Abstract

Schistosomiasis, an immune disease, remains a major public health problem in endemic area. To determine the influence of Nitric Oxide (NO) on this disease, we tested two compounds (Trans-[Ru(bpy)2(NO)SO3](PF6)-PF6 and Na2[Fe(CN)5(NO)]-SNP, which releases NO when activated by biological reducing agents, in BALB/c mice infected subcutaneously by Schistosoma BH strains. The parasitic activity of NO-donors was evaluated in this model by measuring the immune cellular response in liver with: Cytokines levels; histopathological characteristics and the number of the granulomatous lesions; and NO levels. We found that NO-donors treated mice were more resistant to infection, since they exhibited higher survival. Furthermore, we observed in histopathological analysis a decreased influx of inflammatory cells in the hepatic tissue of mice treated with both donors. The parasite counting (estimated as eggs and worms number) was also minor in treated mice. Moreover, decreased levels of IL-10 were detected in the liver of infected mice treated with SNP. The animals treated with PF6 showed high plasmatic NO levels at 45 days after infection. Altogether, these data suggest that NO is a pivotal factor of resistance during schistossomiasis by controlling parasites proliferation, influencing cytokine production and consequently modulating the development of inflammatory response.

Copyright

© 2014 Wander Rogério Pavanelli, Jean Jerley Nogueira da Silva, Carolina Panis, Thiago Mattar Cunha, Francisco José de Abreu Oliveira, Maria Claudia Noronha Dutra de Menezes, Ivete Conchon Costa, Graciele da Silva Thomé, Francisco Ordelei Nascimento da Silva, Eduardo Henrique Silva de Sousa, Luiz Gonzaga de França Lopes, Rubens Cecchini, Fernando de Queiroz Cunha, Eiko Nakagawa Itano and Maria Angélica EharaWatanabe. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.