T-2 Toxin Regulated Ganoderma lucidum Induced Cytokine Release
Kazem Ahmadi and Majid Riazipour
DOI : 10.3844/ajisp.2008.8.13
American Journal of Immunology
Volume 4, 2008
The water-soluble extract of Ganoderma lucidum (Reishi) has been used as immunomodulator to stimulate spleen cells proliferation and cytokine expression. It has also been shown that at some level of exposure, T-2 toxin typically act as immunosuppressive agent and can increase disease susceptibility. The aim of this study was to investigate the effect of T-2 toxin on cytokine production by Ganoderma lucidum (G. lucidum) treated-cells. Mice peritoneal macrophages and lymphoid T cells were prepared by usual manner and plated out at 1×106 or 1×104 cell/well respectively in RPMI 1640 supplemented with 10% FCS, 50 Î¼g streptomycin and 50U penicillin. Cells were incubated with different concentrations of G. lucidum in the presence or absence of 1 ng mL-1 T-2 toxin at 37Â°C and 5% CO2 for 48 h. Cell free medium was removed and used for cytokine assay by ELISA method. The results showed that T-2 toxin in the absence of G.lucidum enhanced IL-2, IFN-g release compared with control group, but it reduced the production of other cytokines. G. lucidum enhanced the production of IL-1b, TNF-a, IL-12, IL-2 and IFN-g compared with control group, but reduced IL-4 and IL-10 release. T-2 toxin, up regulated the enhancement effect of G. lucidum on IFNg, IL-2 and TNF- a, but it down regulated its effect on the production of other cytokines. In conclution our results indicate that T-2 toxin at 1 ng mL-1 may augment the immunomodulating effects of G. lucidum on cytokine release.
© 2008 Kazem Ahmadi and Majid Riazipour. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.