Evaluation of the Immunomodulatory Properties of a Calf Lipid Thymus Extract and Standardization of a Method for Quantification of its Biological Activity
Miodrag Colic, Radmila Popovic, Dusanka Mrdakovic, Milena Radeta, Jasna Novakovic, Bojan Pavlovic and Dragana Vucevic
DOI : 10.3844/ajisp.2007.35.44
American Journal of Immunology
Volume 3, 2007
The aim of this study was to test the immunobiological properties of a new formulation of the lipid thymus calf extract (Thymsol), prepared for in vitro cell culture experiments. At first, we showed that Thymsol enhanced the antigen specific immune response in AO rats in vitro, using Keyhole limpet hemocyanin (KLH). At lower concentrations (0.5-2.5 Î¼g mL-1) the extract increased the proliferation of rat spleen lymphocytes in vitro, stimulated with suboptimal concentrations of Concanavalin A (ConA) or anti-T-cell receptor (TCR) monoclonal antibody (mAb), followed by an increase in the production of interleukin-2 (IL-2). Stimulation of IL-2 production was additionally enhanced in the presence of a blocking anti-IL-2 receptor Î± mAb. Higher concentrations of Thymsol (20-60 Î¼g mL-1) showed a dose-dependent inhibition of lymphocyte proliferation. After testing the variability in IL-2 production by anti-TCR mAb-activated splenocytes, using several stimulatory concentrations of different Thymsol samples, and the production of this cytokine by different cultures of splenocytes treated with the same Thymsol sample, we proposed an IL-2-based method for quantification of the immunobiological activity of the extract.
© 2007 Miodrag Colic, Radmila Popovic, Dusanka Mrdakovic, Milena Radeta, Jasna Novakovic, Bojan Pavlovic and Dragana Vucevic. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.