Towards Identifying Immunogenic Targets in Visceral Leishmaniasis: Role of 17kDa and 63kDa Phosphoproteins
Shubhankar K Singh, Sanjiva Bimal, Diwakar S. Dinesh, Anil K. Gupta, Prabhat K. Sinha, Raajeeva Bimal and Pradeep Das
DOI : 10.3844/ajisp.2005.96.100
American Journal of Immunology
Volume 1, 2005
We observed predominant phosphorylation at Protein Kinase C (PKC-α) residue in seven immunoreactive proteins of virulent Leishmania donovani parasites. These phosphoproteins were tested for their ability to stimulate T-cells for secretion of Macrophage Migration Inhibition Factor (MIF) in comparision to crude soluble antigen in Visceral Leishmaniasis (VL) patients. Two of these phosphorylated L. donovani antigens led Leishmania to present these antigens to Th1 cells, which was obvious from an increased MIF response. These phosphorylated antigens i. e. 63kDa and 17kDa further led to reprogramme CD4 cells for enhanced IFN-γ production. The study suggests that PKC-phosphoryalation in L. donovani antigens can play a critical role in modulation of signal transduction pathway in T-cells to differentiate into Th1 effectors.
© 2005 Shubhankar K Singh, Sanjiva Bimal, Diwakar S. Dinesh, Anil K. Gupta, Prabhat K. Sinha, Raajeeva Bimal and Pradeep Das. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.