Extracts of Pumpkin (Cucurbita pepo L.) Seeds Suppress Stimulated Peripheral Blood Mononuclear Cells in vitro
Christiana Winkler, Barbara Wirleitner, Katharaina Schroecksnadel, Harald Schennach and Dietmar Fuchs
DOI : 10.3844/ajisp.2005.6.11
American Journal of Immunology
Volume 1, 2005
In the traditional medicine in North America and Mexico, pumpkin seeds have been used as an anthelmintic agent and for supportive treatment in functional disorders of the bladder. Also anti-inflammatory and cardioprotective activity of pumpkin seeds is discussed. Three different extracts of pumpkin seeds were prepared and effects were investigated in unstimulated human peripheral blood mononuclear cells and in cells stimulated with the mitogens phytohaemagglutinin and concanavalin A in vitro. Tryptophan degradation and neopterin concentrations were measured in the supernatants allowing to detect biochemical changes induced by cytokine interferon-γ. Extracts of pumpkin seeds suppressed mitogen-induced neopterin production and tryptophan degradation in a dose-dependent way. Data demonstrate capacity of pumpkin extracts to modulate immunobiochemical pathways induced by interferon- γ. Findings imply an immunoregulatory potential of compounds contained in pumpkin seeds.
© 2005 Christiana Winkler, Barbara Wirleitner, Katharaina Schroecksnadel, Harald Schennach and Dietmar Fuchs. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.